Using single particle cryo-electron microcscopy, the Davis lab determines the high-resolution structures of large, macromolecular complexes and uses this information to determine their assembly mechanisms. We also develop new computational tools to facilitate these efforts.
Critically, MIT has made significant investments in this exciting technology, including the purchase of two new microscopes that will be housed in the MIT.nano building, which is scheduled to open in Spring 2018. Additionally, through the MIT Structural Biology Core facility, our group accesses a well-established pipeline to crystallize and determine the structure of proteins of interest.
We recently purchased a top-of-the-line orbitrap mass spectrometer, which is housed in a dedicated mass spectrometry facility. Using this instrument, we can quantitatively determine the composition of large cellular machines, measure the kinetics of their assembly and disassembly and, through the use of cross-linking, gain insights into their overall architecture.
Biochemistry and Biophysics
The Davis lab is well-equipped to purify endogenous macromolecular complexes from yeast and mammalian cell lines as well as to clone, heterologously express and purify proteins of interest in a variety of expression systems. Additionally, we have access to an extensive array of biophysical instruments through the MIT-BIF and the MIT-MicroBio Center including high throughput liquid handling robotics, next-generation sequencing services and analytical equipment such as circular dichroism spectrometers, micro-scale calorimeters and analytical ultracentrifuges.